J0201-3-2 皮膚酵素によるヒト皮膚の抗酸化能の非侵襲センシング(ドラッグデリバリーシステムとその周辺技術の基礎と展開(3))

概要

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When skin is exposed to ultraviolet (UV) light consisting of UVA (320-400nm) and UVB (290-320nm) on the earth. reactive oxygen species (ROS) as well as lipid peroxides and their radicals are formed. It is well documented that these free radicals and ROS cause oxidative cellular stress, cell injury and DNA damage in the epidermis, and eventually induce inflammation, skin photo-aging, photo-toxicity, or malignant tumors. To protect skin from these radical species, there is a multiple natural defense mechanism in the skin. For instance, anti-oxidant enzymes such as superoxide dismutase (SOD). catalase (CAT), and glutathione peroxidase (GSH-Px) play important roles on protecting the skin against degenerative changes by free radicals or ROS. The purpose of this research is to develop an analysis method of CAT, in skin noninvasively by using a test paper. A TIVIBZ was selected as the chromogenic substrate for detecting the catalase activity. The catalase activity ranged between 0 and 4.0 U/ml by using the test paper fabricated. The CVs showed comparatively low level between 2.15 and 2.28 %. It was suggested that the test paper might be used for the quantitative analysis of catalase activity in the stratum comeum.
2009-09-12