2A1-J09 細胞ビルドアップ型ウェットナノロボティクスの構築と機能創発 : 温度応答性培養表面を用いた長期間室温で駆動する昆虫細胞シートの開発

概要

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Here we propose insect heart muscle cell sheets fabrication in order to effectively utilize the cells beating as a driving force of a novel bioactuator. A micro bioactuator utilized a mammalian heart muscle cell sheet was previously reported. But, the actuator requires precise environmental control to maintain the sheet contraction. Therefore, we focused on an insect cell as an alternative driving source, which is generally robust over a range of culture conditions compared to a mammalian cell. In this study, we fabricated insect cell sheets using temperature-responsive culture surfaces for a novel bioactuator. Insect cells dissociated from dorsal vessel tissues which are autonomously beating by tripsin at 4℃ for 24h were contracting more than 2 weeks. Insect cell sheets detached from temperature-responsive culture surfaces by reducing temperature. The insect cell sheets would be utilized as a driving component of an environmentally-robust and autonomous bio-hybrid robotic system.
一般社団法人日本機械学会の論文
2009-05-25