Effects of dissolved oxygen level on cell growth and total lipid accumulation in the cultivation of Rhodotorula glutinis(BIOCHEMICAL ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
The total amount of lipids produced in Rhodotorula glutinis is a subject which has attracted increasing attention due to the potential biodiesel conversion from these microbial oils. The effects of the dissolved oxygen (DO) level in lipid accumulation were examined in this study. Variations of different medium volumes (30, 40 and 50ml) and shaking speed (60, 150 and 210rpm) in the flask trials were adopted to explore the DO effects on lipid production. All of the results revealed that a low DO could retard cell growth, while enhancing lipid accumulation. The 51-fermentor results also confirm that a low DO (25±10%) batch could have higher lipid content than that of high DO batch (60±10%). Nevertheless, the DO level would not obviously affect the lipid composition profile. Oleic acid (C18:1) was the primary fatty acid in both batches. Due to the slow biomass growth rate resulting from the low DO, a two-stage DO controlled strategy (consisting of a high DO stage and following a low DO stage) was performed to improve the cell growth and lipid accumulation simultaneously. However, the strategy was not successful on the enhancement of total lipid production as compared to other batches. Conclusively, even a low DO could retard cell growth; the total production of lipids in the batch with low DO was higher that of the high DO batch due to the enhancement of lipid accumulation. Therefore, the batch operation of R. glutinis at the low DO was suggested for the purpose of lipid production.
著者
-
Zhang Zhiyong
Department Of Dentistry And Oral Surgery Shinshu University School Of Medicine
-
Yen Hong-wei
Department Of Chemical And Materials Engineering Tunghai University
-
Zhang Zhiyong
Department Of Chemical And Materials Engineering Tunghai University
関連論文
- Effects of dissolved oxygen level on cell growth and total lipid accumulation in the cultivation of Rhodotorula glutinis(BIOCHEMICAL ENGINEERING)
- Investigation on Breastfeeding and Lactational Amenorrhoeic Women in Mountainous Areas of Pengxian County, Southwest China
- Osteoinduction with HA/TCP Ceramics of Different Composition and Porous Structure in Rabbits
- Use of poly(ether-block-amide) in pervaporation coupling with a fermentor to enhance butanol production in the cultivation of Clostridium acetobutylicum(BIOCHEMICAL ENGINEERING)
- Using crude glycerol and thin stillage for the production of microbial lipids through the cultivation of Rhodotorula glutinis(BIOCHEMICAL ENGINEERING)
- Effects of dissolved oxygen level on rapamycin production by pellet-form of Streptomyces hygroscopicus(BIOCHEMICAL ENGINEERING)
- Using crude glycerol and thin stillage for the production of microbial lipids through the cultivation of Rhodotorula glutinis