新規癒着防止材熱架橋ゼラチンフィルムの物理的・生物学的特性 : 癒着防止効果の機序及び腸管吻合部への影響に対する基礎的検討

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To prevent postoperative abdominal adhesion, we have developed a novel anti-adhesive material made of thermally cross-linked gelatin film. Previously, we reported that it had an excellent anti-adhesive effect and that it could be used safely for intestinal anastomosis in a canine model. This study analyzed the physical and biological properties of the thermally cross-linked gelatin film preliminarily, and investigated the handling and the mechanism of anti-adhesive effect and the influence on intestinal anastomotic sites, in comparison to a combination film composed of hyaluronic acid and carboxymethyl cellulose (cellulose film), that is commonly used in clinical situations. The tensile strength of the thermally cross-linked gelatin film was significantly higher than that of cellulose film when twisted to 90° in both wet and dry conditions. A degradation test in distilled water showed both films dissolved gradually with time and were nearly 70-80% dissolved by seven days. This suggested that these films have similar solubility. Tests of in vitro cell growth and viability on each film showed that cultured cells grew well over time on the thermally cross-linked gelatin film. On the other hand, the cell number decreased significantly on cellulose film and most of the cells died within seven days. These results suggest the thermally cross-linked gelatin film had more physical strength and better handling than cellulose film. Both thermally cross-linked gelatin film and cellulose film were thought to prevent adhesion as physical barrier during seven days after surgery, which is a significant period for adhesion formation. However the results also suggested that the cellulose film acted as anti-adhesive membrane by inhibiting cell growth and wound healing. In contrast, the thermally cross-linked gelatin film did not inhibit cell growth, and resulted in more favorable wound healing as well as anti-adhesive effects. Therefore, it could be used safely for intestinal anastomosis. In conclusion, the thermally cross-linked gelatin film is considered to be an excellent anti-adhesive material that has no negative effects on intestinal anastomosis sites.

新規癒着防止材熱架橋ゼラチンフィルムの物理的・生物学的特性 : 癒着防止効果の機序及び腸管吻合部への影響に対する基礎的検討

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