Study on the expression of renal ACE, ACE2 of surgery hypertensive rats(Basic medicine)

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Objective Modeling a surgery hypertensive rat model, observe the changes of the expression of ACE and ACE2 in various groups, thus for further research of the pathogenesis of hypertension. Methods Twenty-eight Sprague- Dawley rats were used, which were divided randomly into four groups. One group served as control (n=7), others as hypertensive group (n=7), enalapril group [n=7,15mg/(kg・d)] and losartan group [n=7,15mg/(kg・d)]. All rats were operated as surgery hypertension except rats of control group. Renal artery was ligated. Enalapril and losartan were used as treatments of rats of enalapril group and losartan group after surgery. Blood pressure of rats was measured by arteria caudilis uninjured. The expression of renal ACE and ACE2 mRNA were measured by RT-PCR. The expression of renal ACE and ACE2 protein were measured by immunohistochemistry. Results Blood pressure of rats was increased after operation. The expression of ACE and ACE2 mRNA and protein in various groups were different. The expression of ACE mRNA and protein of Hypertensive group were higher than others. The expression of ACE2 mRNA and protein of losartan group were higher than others. Conclusion Model of surgery hypertensive rats were success by the ligation of renal artery. It's a Simple, reliable and cheap method of hypertensive rat model. The imbalance between the expression of ACE and ACE2 is one of the important roles of pathogenesis of hypertension.
2011-03-30