Extraction and in vitro antioxidant activity of intracellular polysaccharide by Pholiota adiposa SX-02(BIOCHEMICAL ENGINEERING)

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Response surface methodology (RSM) was used to optimize the extraction parameters for Pholiota adiposa SX-02 intracellular polysaccharide (IPS) produced during submerged culture. The optimum conditions of IPS extraction were predicted to be, ultrasonic power at 564.93W, precipitation time 30.34h and pH 8.28, and IPS yield was estimated at 19.75%. The actual value of IPS under these conditions was 20.51%. The in vitro antioxidant results showed that the inhibition effects of IPS at a dosage of 250mg/l on superoxide anion, hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical were 74.66±5.31%, 69.20±5.13%, and 75.20±6.73%, respectively, which were 27.51±2.23%, 16.58±1.33%, and 9.46±0.72% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of IPS was 0.32±0.02 (absorbance at 700nm), 39.13±3.47% higher than that of BHT. The results provide a reference for large-scale extraction of IPS by P.adiposa SX-02 in industrial fermentation and the IPS can be used as a potential antioxidant which enhances adaptive immune responses.
社団法人日本生物工学会の論文