cDNA Cloning, Genomic Structure and Expression Analysis of the Bovine Lanosterol 14α-Demethylase (CYP51) in Gonads(Molecular and Cell Biology)

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概要

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• Meiosis activating sterol (MAS), the intermediate of cholesterol biosynthesis, is an important substance to stimulate oocytes maturation in FSH-induced signal transduction pathway. Lanosterol 14α-demethylase (CYP51) converts lanosterol to MAS. Although MAS is firstly isolated from bovine testis, the information about bovine CYP51 gene and its expression is little. In present studies, the cDNA cloning, genomic structure, chromosomal mapping, and expression patterns of bovine CYP51 were demonstrated. The cDNA coding bovine CYP51 contains a 1509bp open reading frame and a 1119bp 3' untranslated region. And the bovine CYP51 genen includes 10 exons and spans about 17 kb. Screening the cattle RH_<5000> panel bovine CYP51 is mapped to chromosome 4 (0cR). The sequenced promoter region is TATA-less and contains several highly conserved regulatory elements, such as GC-box, cAMP-responsive elements (CRE), sterol regulatory element (SRE) which is important fragment for its transcription. No evidence of processed pseudogenes is found using long PCR and Southern blot. Northern blot analysis reveals that an approximately 2.7 kb mRNA is expressed in all the examined bovine tissues, while a 1.8 kb mRNA is found only in the mature bovine testis where the MAS is accumulated. Immunochemistry analysis shows that leydig cells express the highest level of the CYP51 protein in testis. Among different stages follicles it is localized primarily to the oocytes with the level varying slightly. Granulosa cells of primordial, primary and secondary follicles show background staining. While granulosa cells facing the antrum and cumulus granulosa cells of antral follicles show considerably heavier staining. The highest level is expressed in corpus lutea. These data indicate a stage- and cell type-specific expression of CYP51 protein in bovine oogenesis.

• 公益社団法人日本薬学会の論文
• 2006-03-01

著者

• XIA Guoliang

  College of Biological Sciences, China Agricultural University

• Xia Guoliang

  College Of Biological Science China Agricultural University

• Zhou Bo

  College Of Biological Science China Agricultural University

• Shen Yue

  College Of Biological Science China Agricultural University

• Wang Fengchao

  College Of Biological Science China Agricultural University

• SONG Xiaoming

  College of Biological Science, China Agricultural University

• CHEN Xiu

  Heze University

• LEI Lei

  College of Biological Science, China Agricultural University

• Song Xiaoming

  College Of Biological Science China Agricultural University

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• cDNA Cloning, Genomic Structure and Expression Analysis of the Bovine Lanosterol 14α-Demethylase (CYP51) in Gonads(Molecular and Cell Biology)
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