In vivo optical measurements of cortical response evoked by electrical stimulation in the mouse visual cortex (MEとバイオサイバネティックス)

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Electrical stimulation applied to visual cortex is known to evoke phosphenes in blind patient, indicating a possibility of cortical implant. It is essential to understand spatio-temporal properties of the cortical responses evoked by electrical stimulation to visual cortex in order to design the implant electrodes. We conducted optical measurement of the mouse visual cortex activity in vivo using voltage-sensitive dye (VSD). Abiphasic current stimulation was delivered to primary visual cortex (VI) by glass microelectrode. Intracortical stimulation (>40 μA) induced response around the electrode that spread within V1 and to nearby area, including latero-medial (LM) area. The amplitude and the area of response became larger as the stimulation current. Stimulation on the dura and superficial layer (layer 1) was less effective. The stimulation on deeper layers, namely layer 5 and 6 and white matter layer, induced larger and widely spreading activity that was followed by long lasting inhibitory response.
2010-03-02