Preparation of arrays of cell spheroids and spheroid-monolayer cocultures within a microfluidic device(CELL AND TISSUE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
This study describes a novel method for generation of an array of three-dimensional (3D) multicellular spheroids within a microchannel in patterned cultures containing one or multiple cell types. This method uses a unique property of a cross-linked albumin coated surface in which the surface can be switched from non-adhesive to cell adhesive upon electrostatic adsorption of a polycation. Introduction of a solution containing albumin and a cross-linking agent into a microchannel with an array of microwells caused the entire surface, with the exception of the interior of the microwells, to become coated with the cross-linked albumin layer. Cells that were seeded within the microchannel did not adhere to the surface of the microchannel and became entrapped in the microwells. HepG2 cells seeded in the microwells formed 3D spheroids with controlled sizes and shapes depending upon the dimensions of the microwells. When the albumin coated surface was subsequently exposed to an aqueous solution containing poly (ethyleneimine) (PEI), adhesion of secondary cells, fibroblasts, occurred in the regions surrounding the arrayed spheroids. This coculture system can be coupled with spatially controlled fluids such as gradients and focused flow generators for various biological and tissue engineering applications.
著者
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KHADEMHOSSEINI Ali
Harvard-MIT, Division of Health Science and Technology
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Okuyama Tomoaki
Graduate School of Pure and Applied Sciences, University of Tsukuba
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Yamazoe Hironori
Nanotechnology Research Institute, Advanced Industrial Science and Technology
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Suzuki Hiroaki
Graduate School of Pure and Applied Sciences, University of Tsukuba
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Fukuda Junji
Graduate School of Pure and Applied Sciences, University of Tsukuba
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Mochizuki Naoto
Graduate School of Pure and Applied Sciences, University of Tsukuba
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Fukuda Junji
Graduate School Of Pure And Applied Sciences University Of Tsukuba
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Okuyama Tomoaki
Graduate School Of Pure And Applied Sciences University Of Tsukuba
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Khademhosseini Ali
Harvard-mit Division Of Health Sciences And Technology Massachusetts Institute Of Technology:departm
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Yamazoe Hironori
Nanotechnology Research Institute Advanced Industrial Science And Technology
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Mochizuki Naoto
Graduate School Of Pure And Applied Sciences University Of Tsukuba
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Suzuki Hiroaki
Graduate School Of Pure And Applied Sciences University Of Tsukuba
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Fukuda Junji
Dep. Of Life And Environment Engineering The Univ. Of Kitakyushu 1-1 Hibikino Wakamatsu-ku Kitakyush
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Suzuki Hiroaki
Graduate School Of Information Science And Technology Osaka University
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Fukuda Junji
Graduate School Of Pure And Applied Sciences Univ. Of Tsukuba
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