Cell Microarray for Screening Feeder Cells for Differentiation of Embryonic Stem Cells(CELL AND TISSUE ENGINEERING)
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概要
- 論文の詳細を見る
Microarrays are currently recognized as one of major tools in the assessment of gene expression via cDNA or RNA analysis and are now accepted as a powerful experimental tool for high-throughput screening of a large number of samples, such as cDNA and siRNAs. In this study, we examined the potential of the microarray methodology for high-throughput screening of candidate cells as feeder cells which effectively differentiate embryonic stem (ES) cells to the specific lineage. Cell arrays were prepared by applying three kinds of cells, PA6, human umbilical vein endothelial, and COS-1 cells, to circular spots, 2mm in diameter, on a glass plate, followed by the application of mouse ES cells to the cell microarray. After 8 d in culture, TuJ1 (neuron-specific class III β-tubulin) immunocytochemical staining clearly demonstrated that only PA6 cell spots had the capability to induce ES cells to neuronal differentiation. Although this is a model experiment, these findings clearly indicate that the cell microarray will become a powerful tool for high-throughput screening large numbers of candidate feeder cells for specific differentiation.
- 社団法人日本生物工学会の論文
- 2005-09-25
著者
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Iwata Hiroo
Institute For Frontier Medical Sciences Kyoto University
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Yamazoe Hironori
Nanotechnology Research Institute Advanced Industrial Science And Technology
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Yamazoe Hironori
Institute For Frontier Medical Sciences Kyoto University
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Iwata Hiroo
Institute For Frontier Medical Science Kyoto University
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