78.イネの病原菌認識時に誘導される活性酸素発生のCa2+依存性プロテインキナーゼによる制御機構解析(口頭発表)

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One of the earliest plant immune responses is the oxidative burst, in which levels of reactive oxygen species (ROS) rapidly increase. The oxidative burst are regulated by Rboh (respiratory burst oxidase homolog) which classified in NADPH oxidase. It suggests that Rboh is regulated by calcium-dependent protein kinase (CPK). CPK is serine-threonine kinase and has EF hand motifs to interact with Ca^<2+>. However, It is little known how CPK regulates the activation of Rboh. I try to reveal that CPK is involved in regulation on the oxidative burst. We identified 29 CPKs from rice genome and expression of 6 CPKswere induced during the plant immune responses. We made 6 CPKRNAi knock down mutants and OsCPK12 RNAi knock-down mutant lost the ability of oxidative burst. OsCPK12knock-out mutants also had no activity of ROS generation after avirulent pathogen recognition. BiFC analysis using OsCPK12 and Osrbohs showed constituted active OsCPK12 interacted with N terminal domain of OsrbohA. Because dominant negative OsCPK12 did not interact with any domain of Osrbohs, interaction between OsCPK12 and OsrbohA was depended on Ca^<2+>-dependent activation of OsCPK12.
2010-10-01