77.植物病原細菌の鞭毛タンパク質フラジェリンのイネにおける特異的認識に関与する受容体の同定とその認識機構解析(口頭発表)

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Rice has perception systems of flagellin from rice-incompatible strain of phytopathogenic bacterium Acidovorax avenae, which induced immune responses. However, the receptor for the flagellin in rice is still not known. FLS2 receptor in Arabidopsis recognized flg22, a peptide derived from the flagellin N-teminus. To clarify whether the OsFLS2, rice ortholog of FLS2, is involved in the recognition of the flagellin from A. avenae avirulent strain in rice. The OsFLS2-RNAi plants still maintained the flagellin recognition ability, suggesting that OsFLS2 is not main receptor to flagellin in rice. To identify the active region of flagellin recognized by rice, various domains in flagellin were produced and examined immune response activity. Flagellin C-terminal domain was only induced the immune responses in rice. Gene expression profiling during flagellin treatment showed that expression of 18 genes encoding the receptor kinase were increased. Among the identified genes, Flagellin-induced Receptor Kinase 1(FliRK1) complemented the flagellin recognition ability in fls2 mutant. Two flirk1 knock-out mutants showed the insensitive phenotype to flagellin. Next we examined the direct interaction between flagellin and FliRK1 using FliRK1-overexpressing Arabidopsis protoplasts. Immune precipitation analysis revealed that flagellin directly interacted with FliRK1, suggesting that FliRK1 is specific receptor for flagellin in rice.
2010-10-01