スギ花粉アレルゲン(Cry j 1,Cry j 2)の共通抗原性

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Patients with pollinosis caused by Japanese cedar (Cryptomeria japonica) pollen often have additional allergy to the pollen of Hinoki (Chamaecyparis obtusa). This phenomenon is due to cross-allergenicity between Cry j 1, the allergen from C. japonica pollen, and Cha o 1, the allergen of C. obtusa pollen. In this study we have surveyed pollen of various species for signs of cross-allergenicity with the allergens Cry j 1 and Cry j 2 from C. japonica. Mature pollen grains from a range of woody dicotyledonous species were examined by the enzyme linked immunosorbent assay (ELISA) using the anti-monoclonal antibody (anti-mAb) 013 against the allergen Cry j 1, and anti-mAb T27 against the allergen Cry j 2. Anti-mAb 013 reacted with Morus bombycis pollen twice as strongly as with C. obtusa pollen; it also reacted weakly with pollen from Sambucus sieboldiana, Betula platyphylla, Mallotus japonicus and Myrica rubra. However, Anti-mAb T27 only reacted with B. platyphylla pollen. Moreover, immunocytochemical localization of Cry j 1- and Cry j 2-like proteins was examined in the pollen of these species, and showed cross-reactivity in ELISA. Anti-mAb 013 showed marked reactivity with the orbicules, exine, cytosol, and starch granules in the pollen of C. obtusa and B. platyphylla but only weak reactivity with the pollen of the other species. Anti-mAb T27 only reacted with the pollen of B. platyphylla and C. obtusa. These results suggest that as well as C. obtusa pollen, B. platyphylla pollen may have cross-allergenicity with C. japonica pollen.
2010-06-30

スギ花粉アレルゲン(Cry j 1,Cry j 2)の共通抗原性

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