Thermostable Kunitz trypsin inhibitor with cytokine inducing, antitumor and HIV-1 reverse transcriptase inhibitory activities from Korean large black soybeans(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

概要

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A large number of trypsin inhibitors belonging to various types have been purified from different kinds of legumes. In this study, by using liquid chromatography, a Kunitz type trypsin inhibitor (KBTI) with a molecular weight of 20107.645 Da was purified from Korean large black soybeans. KBTI reduced the proteolytic activities of trypsin and α-chymotrypsin with the activity of 〜8520 BAEE units/mg and 〜24 BTEE units/mg, respectively. It showed high thermal stability (0-100℃) as well as stability over a large range of pH values (pH 3-11). Furthermore, KBTI inhibited HIV-1 reverse transcriptase activity with an IC_<50> value of 0.71μM and induced the release of pro-inflammatory cytokines such as TNF-α, IL-1β, IL-2 and interferon-γ at the mRNA level. KBTI exerted weak antiproliferative activity toward CNE-2 and HNE-2 nasopharyngeal cancer cells, MCF-7 breast cancer cells, and Hep G2 hepatoma cells. KBTI was destitute of mitogenic ribonuclease and antifungal activities.