Campesin, a thermostable antifungal peptide with highly potent antipathogenic activities(MISCELLANEOUS)

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概要

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• An 9.4-kDa antifungal peptide designated as campesin was isolated from seeds of the cabbage Brassica campestris. The isolation procedure involved affinity chromatography on Affi-gel blue gel, ion exchange chromatography on Q-Sepharose and Mono S, and gel filtration on Superdex 75 and Superdex Peptide. The peptide was adsorbed on the first three chromatographic media. It exerted an inhibitory action on mycelial growth including Fusarium oxysporum and Mycosphaerella arachidicola, with an IC_<50> of 5.1μM and 4.4μM, respectively. The peptide was characterized by remarkable thermostability and pH stability. It inhibited proliferation of HepG2 and MCF cancer cells with an IC_<50> of 6.4μM and 1.8μM, and the activity of HIV-1 reverse transcriptase with an IC_<50> of 3.2μM. It demonstrated lysolecithin binding activity.

著者

• Lin Peng

  Department Of Endocrine Qilu Hospital Shandong University

• Lin Peng

  Department Of Biochemistry Faculty Of Medicine The Chinese University Of Hong Kong

• Wong Jack

  School Of Biomedical Sciences Fac. Of Medicine The Chinese Univ. Of Hong Kong Shatin New Territories

• Wong Jack

  Department Of Biochemistry Faculty Of Medicine The Chinese University Of Hong Kong

• Ng Tzi

  School Of Biomedical Sciences Fac. Of Medicine The Chinese Univ. Of Hong Kong Shatin New Territories

• Xia Lixin

  College of Life Science, Shenzhen University

• Ng Tzi

  Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong

• Xia Lixin

  College Of Life Science Shenzhen University

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