組換えGFP(緑色蛍光タンパク質)を用いたタンパク質の学習プログラムの開発

概要

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Green Fluorescent Protein (GFP) is a protein purified from the jellyfish, Aequorea victoria. The modifiedGFP gene has been inserted into pGLO plasmid and now is available from Bio-Rad Laboratories for educationalapplication of studies on biotechnology or molecular biology. Recombinant GFP expressed in E.coli transformedwith pGLO is a highly potent tool for "protein literacy". We proposed an experimental protocol constituted of afew GFP purification methods, which are useful for high-school students and undergraduates to learn thebiochemistry of proteins, and introduced an example of the practice in the school.