低温保存および凍結保存同種血管移植後の拒絶反応における15-Deoxyspergualinの効果 : 病理組織学的および免疫組織化学的検討

概要

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In this study, the effect of 15-deoxyspergualin on immunological rejection were evaluated after aortic allograft implantation in graft homovital-preserved group and graft-cryopreserved group. Descending aorta harvested from Wistar-King rats and preserved with either homovital preservation (4℃) or cryopreservation (-196℃) for two weeks were heterotopically implanted to the abdominal aorta of the recipient, Lewis rats. After implantation, 15-deoxyspergualin was administered intraperitoneally at the dose of 10 mg/kg/day for a month. Histological and immunohistochemical changes were assessed one month after implantation among the four groups. In the cryopreserved group, endothelial cells and fibroblast were well-preserved histologically. Immunohistochemical stains indicated that remarkable deposition of IgG, IgM and C3, especially IgM, existed in control group of no treatment with 15-deoxyspergualin, while the extent of the same deposition was significantly less in the 15-deoxyspergualin-treated groups. Our results indicated that the viability of cryopreserved aortic allograft could be sufficiently maintained for within two weeks and the inhibitory effect of 15-deoxyspergualin on immunological response and rejection was observed.
東京女子医科大学の論文
2000-05-25