蛋白構造と機能予測のためのアミノ酸配列解析法の開発

概要

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In order to study the relationship between amino acid sequences and protein functions, we developed regional estimation methods for given amino acid sequences using the distribution (REMAD) and the chemical characters (REMAC) of amino acids. The REMAD searches for amino acid locations in the given sequence and then visualizes the distribution graphically for any selected amino acid. The REMAC calculates the average of desired character-values in a set of fixed size stretches of given sequence. We verified these methods using the test sequences and a human cardiac β-myosin heavy-chain peptide (cardiac β-MHC). Then we applied these methods to wild type and mutants of a cardiac β-MHC. In the cardiac β-MHC, REMAD showed a clear difference in the amino-acid distribution of the head and rod regions of the sequence. We found out the characteristic sequence (XX 7 amino acids X:X shows any of G, F, M, Y, P) of head region. REMAC with hydrophobicity parameters showed results implicating the potential relationship between the hydrophobic character of the peptide, force generation and sliding velocity. These results clearly indicate that the present methods have the capacity to suggest the functionality of proteins from their amino acid sequences.
東京女子医科大学の論文
2001-01-25