LECTIN BINDING SITES OF TRYPANOSOMAGAMBIENSE

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概要

• 論文の詳細を見る

• Intact Trypanosoma gambiense was not agglutinated by plant lectins; concanavalin A (Con A), wheat germ agglutinin (WGA), soybean agglutinin (SBA), phytohemagglutinins M and P (PHA-M, PHA-P), ricinus communis agglutinin-I (RCA-I), and peanut agglutinin (PNA). However, the parasites were agglutinated specifically by low concentrations of these lectins after brief treatment by trypsin, chymotrypsin, papain, chymopapain, or pronase. On the other hand, ulex europaeus agglutinin-I (UEA-I) and dolichos biflorus agglutinin (DBA) did not induce the agglutination of either the intact or enzyme-treated parasites. Dextranase and α-amylase treatment of the protease- digested parasites did not reduce agglutination by lectins. Aggulutination with lectins did not occur when an inhibitory concentration of a competitive sugar was present. Results indicated that lectin-binding sites on the cell surface of T. gambiense were not directly associated with α-1, 4 or α-1, 6 glycosidic bonds of polysaccharides. Con A sites on the cell surface were visualized at the fine structure level with a lectin-ferritin conjugate. The electron-dense ferritin particles were distributed randomly on the enzyme- treated cell surface and flagella membrane. Con A bound at the cell surface was also visualized with horseradish peroxidase (HRP) and diaminobenzidine (DAB)-coupled reactions. Dense Con A-HRP-DAB reaction product was deposite uniformly over the entire cell surface and flagella membrane. Localization of WGA and SBA bound at the T. gambiense cell surface was also facilitated using HRP-DAB-coupled reactions. The fine structure distribution of these HRP-conjugated lectins was similar to that obtained with Con A-HRP-DAB preparations. Both the living and glutaraldehyde-fixed parasites gave similar agglutination results with Con A. The parasites also showed strong agglutination by Con A at low temperature. Colchicine- and cytochalasin B-preteated parasites also showed marked agglutination by Con A. These results suggest that parasite agglutination by Con A differs from mammalian cell agglutination, since clustering of Con A-binding sites is not necessary for parasite agglutination.

• 名古屋市立大学の論文
• 1981-11-30

著者

• 籔 義貞

  Department Of Medical Zoology Nagoya City University Medical School

• 藪 義貞

  名古屋市立大学 医動物

• 藪 義貞

  Department of Medical Zoology, Nagoya City University Medical School

関連論文

• In vitro cultivation system for Trypanosoma gambiense bloodstream forms with mouse fibroblast-like cells
• SUCCINYL CON A-INDUCED ADDLUTINATION OF TRYPANOSOMA GAMBIENSE
• LECTIN BINDING SITES OF TRYPANOSOMAGAMBIENSE

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