PROTEINS INVOLVED IN CONNECTION OF SYNAPTIC JUNCTIONAL COMPONENTS OF RAT BRAIN AND THEIR DEVELOPMENTAL CHANGE

概要

論文の詳細を見る
The present work investigated the characteristics and function s of the protein constituents that composed synaptic junctions of rat cerebrum and cerebellum. (1) The analysis of the developmental changes in cerebellar synaptic junctions by both electron microscopy and polyacrylamide gel electrophoresis suggested that at least one of the proteins (>300 k, 240 k, 230 k, 120 k, and 110 k Mr) was localized in the synaptic plasma membrane or in its adjacent area, and involved in the linkage of the postsynaptic density to the synaptic membrane. (2) The pre- and post-synaptic structures of the cerebrums were separated by trypsin treatment. The comparison of the electrophoretic analysis of junctional components indicated that one or some of the proteins (240 k, 230 k, 200 k, 190 k, 180 k, 145 k, 110 k, 105 k, 55 k, 51 k, and 45 k Mr), glycoproteins (190 k, 135 k, 105 k, 110 k, 72 k, 55 k, 51 k, and 24.5 k Mr), and Con A-binding proteins (180 k and 130 k Mr) might be involved in the adhesion mechanism between pre- and post-synaptic structures. (3) It wsa interpreted, from the effects of neuraminidase and glycopeptidase on cerecral synaptosomal fractions, that carbohydrate fragments separable from glycoproteins in the synaptic plasma membrane by these enzymes took no part in synaptic conection.
名古屋市立大学の論文
1982-12-25