(2)中枢神経系に存在する生理活性物質Pituitary Adenylate Cyclase-Activating Polypeptide(PACAP)によるゴナドトロピン産生調節(<特集>第61回学術講演会シンポジウム2「中枢神経関連生理活性物質の生殖機能へのかかわり」)

概要

論文の詳細を見る
We examined how pulsatile stimulation with Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) affected gonadotrophs. In static culture, GnRH stimulated transcription of all of the gonadotropin subunits. In contrast, PACAP increased α-subunit promoter activity, but failed to increase LHβ and FSHβ promoters. Messenger RNAs for LHβ and FSHβ were slightly but significantly increased by PACAP stimulation. Co-treatment of the cells with GnRH and PACAP was not different to the effects of GnRH alone on LHβ and FSHβ transcriptional activities as well as mRNA expressions. To determine the effect of pulsatile PACAP stimulation on gonadotropin subunit gene expression, perifused LβT2 cells were stimulated at either high frequency (5min PACAP pulse every 30min) or low frequency (5min PACAP pulse every 120min). High frequency PACAP pulses preferentially increased LHβ gene expression up to 2.29±0.15-fold, and low frequency pulses resulted in a 1.55±0.16-fold increase. FSHβ gene expression was increased 1.87±0.3-fold by high frequency PACAP pulses and 4.3±0.29-fold by low frequency pulses. These results were similar to the frequency specific effects of pulsatile GnRH. Follistatin expression was specifically increased by high frequency GnRH pulses. High frequency PACAP pulses further increased follistatin up to 4.7±0.57-fold than low frequency pulse (2.72±1.09-fold). PAC1 expression increased significantly following pulsatile GnRH regardless of pulse frequency. Low frequency PACAP pulses, however, increased PAC1 expression (16.49±8.41) to a larger extent than did high frequency pulses. Additionally, high frequency PACAP pulses specifically increased GnRH receptor expression by 4.38±0.81-fold; however, low frequency pulses did not result in an increase. These studies suggest PACAP, like GnRH, specifically regulates LHβ and FSHβ subunit gene in a pulse-frequency specific manner. This regulation may involve alteration in numbers of GnRH and PACAP receptors as well as follistatin expression.
社団法人日本産科婦人科学会の論文
2009-10-01

(2)中枢神経系に存在する生理活性物質Pituitary Adenylate Cyclase-Activating Polypeptide(PACAP)によるゴナドトロピン産生調節(<特集>第61回学術講演会シンポジウム2「中枢神経関連生理活性物質の生殖機能へのかかわり」)

スポンサーリンク

概要

著者

関連論文

スポンサーリンク