16. Disc-in-Bag法による酵母菌株の乾燥保存(昭和56年度第27回凍結及び乾燥研究会特別講演および研究報告)

概要

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Seventy nine yeast cultures of 53 different species belonging to 16 genera have been dried by a disc-in-bag method for preservating microbial cultures. Yeast cells were suspended in protective agent (serum broth), dropped on polyethylene foil, and dried in a laminar air flow bench for several hours. Dried discs were stored in a desiccator at room temperature for 51 to 56 months. After preservation, viable counts were carried out. With the exception of the death of only one strain, Torulopsis halonitratophila, all the other strains survived well. Of the 79 cultures tested 28 (35.4%) ranged from 10^7 to 10^8 viable cells per disc, and 49 (59.5%) ranged from 10^4 to 10^8 viable cells per disc. Morphological characteristics, fermentation reactions, assimilation of carbon compounds and nitrates, and growth temperatures of the 40 isolated cultures representing 11 genera after storage for 51 to 56 months, were compared with the data which was examined before drying. Cultures which were preserved have unalterably maintained the morphological and physiological characteristics they showed at the time of their original isolation.
低温生物工学会の論文
1981-09-20