8. ラット摘出心臓の保存・蘇生・移植(セミナー「低温と乾燥による生物保存技術への挑戦〜マミーエンジニアリング」)

概要

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The inert fluid perfluorocarbon (PFC) has been used since about 1960 in liquid respiration and artificial blood for mammals. PFC has been used to successfully resuscitate tardigrades that had been dried and exposed to a high barometric pressure of 6,000 atmospheres. Next, scientists attempted to experimentally preserve organs that had been removed from animals, dried, and immersed in PFC. Since 1998 preservation and resuscitation experiments have been conducted with mammalian hearts using 2,015 rats and 70 pigs. Among those experiments, the maximum time after desiccation until successful resuscitation was 26 days for a rat heart and 37 days for a pig heart. However, these results could not be reproduced. Finally, in 2005, this laboratory demonstrated that, a rat heart removed under of 2 atmospheres pressure and a CO_2 partial pressure of 400 hPa, followed by desiccation for 24 hr, could be revived and heterotypically transplanted. Moreover, these results were reproducible. The preservation time can be extended to 72 hr if, after immersing isolated rat hearts in PFC, they are dried by air exposure under a CO_2 partial pressure of 100 hPa. The present report documents the resuscitation of this heart after 48-120 hr of preservation followed by heterotrophic transplantation.
低温生物工学会の論文
2008-08-21