肺結核患者の気管内細菌叢の研究(第2報)
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Non-pathogenic bacteria except tubercle bacilli were isolated from the materials of intrabronchial exudate in 30 cases by Brbnchoscopy and of cavities resected from 10 cases of tuberculosis patients. Development of resistance of the isolates to the antibiotics which were used for therapy was also tested. The results obtained were summarized as follows. 1. Bacterial flora in bronchial tubes were chiefly Streptococcus, Micrococcus, Sarcina, Mycococcus, Nocardia and Yeast. Gram negative rods were seldom isolated. 2. Streptococcus as well as staphylococcus were always dominant when human whole blood was added in media. In many cases, red or pink pigmented Micrococcus were isolated (20 cases out of 30). Those organisms were idantified as Micrococcus rhodochrous and Micrococcus cinnabareus which were recently rearranged as Mycococcus by krassilnikov. 3. Aboundant colonies of Nocardia and Nocardia like organisms were found in plates when the human blood media were used. After much mycological investigation, those organisms were classified into 4 species of Nocardia. 4. Viable bacterial count of per gram of the branchial fluid was carried out. In one case, 2,200×10^3 bacteria were counted, while 63×10^3 in the other case. 5. The isolated Gram positive cocci such as red and yellow Mycococcus, Staphylococcus and Sarcina were resistant to streptomycin, growing well at the concentration of 1,000 meg/ml of the drug. It is certain that the development of resistance, to the drug is due to long run therapy of streptomycin. On the other hand, the strains of Nocardia and Streptococcus were rather sensitive to Streptomycin. All bacteria were also sensitive to chlortetracycline. 6. In the isolation of becteria from tuberculous cavities, both gram positive Micrococcus and negative diplococcus were detected from only 3 cases out of 21 cavities. Those isolates showed high resistance to SM.
- 千葉大学の論文
- 1959-09-28
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