回虫卵の発育に関する基礎的研究 : 第1編ホールグラス寒天培養法による同一回虫卵の継続観察の一方法について

概要

論文の詳細を見る
37℃ with 100% humidity. The following observations were made: 1. This "hole glass agar-agar culture method" has various advantages. It has a high incidence of production of embryonated eggs, and attains fairly constant results at all times; it needs no skill to carry out the procedure after inoculation of eggs; microscopic pictures can be taken serially at any time, and slides can be long preserved; and observations can be made of the development of a large number of different batches of eggs continually in chronological sequence. 2. The numbers of days required for the eggs to reach the embrionated stage on the average were: 9.97 ± 0.40 days for Ascaris lumbricoides, and 8.44±0.55 days for A. suilla, at 29℃; 23.67±1.14 days for A. suilla at 21℃. 3. When incubated at 37℃, the eggs of A. suilla onld reached the segmentation stage, none of them showing any further developments. 4. Three different batches of Ascaris eggs, after having been kept in a refrigerator for 25 days, 40 days and 174 days, respectively, were incubated at 29℃ in the same way. The results have shown that there are no differences in the speed of development between the three different batches.
千葉大学の論文