鉤虫卵及び東洋毛様線虫卵の培養法に関する研究

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Several factors which, being present in the feces and methods of culture, might have influence on the development of Ancylostoma and Trichostrongylus orientalis are investigated. The result is summarized as follows : , 1. A study of the eggs of Necator americanus, Ancylostoma duodenale and Trichostrongylus orientalis in the feces left standing in a room temperature (19℃ on an average) and in a low temperature (0.3℃ on an average) revealed that the survival curve followed by Trichostrongylus orientalis was the most gentle, followed by the survival curves representing Ancylostoma duodenale and Necator americanus in the indicated order. Low temperature did a great deal of injuries to the eggs of all three kinds of worms. 2. Considering that some eggs may perish if the feces containing them are left standing for a long while, it is desirable that the sample of feces used for measurements of the numbers of worms is as fresh as is obtainable. In view of the fact, however, that at least some of the larvae of these three kinds of worms survived a low temperature for ten days, the eggs of worms present in the feces would be identifiable even if they are left standing for a while. 3. If the surface of a rough tile to be smeared with a sample of the feces is about 7.5cm. in diameter, the proper amount of a sample to be used would be from 0.1gm. to 0.3gm. However low the density of the distribution of worm eggs in the feces may be, they are sure to be detected upon culture, if this amount of feces is used. 4. Experiments performed in order to see if dilutions of some disinfectants, antiseptics and antibiotics have any advantage over filtered water as a medium to be used for the culture of these worm eggs revealed that a good result could be obtained by seeding worm eggs in filtered water, unless germ-free culture is what the investigator has in view. 5. Comparison of Kawara (rough tile) culture method with filter paper method revealed that the tormer always produced larger numbers of larvae than the latter. 6. The author has developed erect Kawara culture method in which are incorporated the virtues of the above two methods. 7. There is no choice between erect Kawara culture method and Kawara culture method so far as the number of larvae developed is concerned. However, the former is superior to the latter in that it is easier to operate. Erect Kawara culture method would, therefore, be useful in mass examinations to detect parasite worm eggs.
千葉大学の論文
1958-05-28

著者

大野俊雄
千葉大学医学部公衆衛生学教室

鉤虫卵及び東洋毛様線虫卵の培養法に関する研究

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