鉤虫卵及び東洋毛様線虫卵の培養法に関する研究
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Many methods have long been employed for the culture and hatching of the eggs of Ancylostoma and Trichostrongylus orintalis. As a result of the study of these methods of culture, especially Kawara (rough tile) culture method and filter paper culture method which are now widely used, the author has developed erect Kawara culture method which incorporates the virtues of these two culture methods. The present paper consists of four parts. The part I deals with a general study of the factors involved in the development and hatching of hookworm eggs. These factors were studied from the viewpoint of ecology after they have been classified into the following four groups: the factors inherent in parent worms, in eggs, in feces and in method of culture. As a preliminary to comparative study of various culture methods to be undertaken for the purpose of evaluating .them on the basis of the number of larvae hatched, the number of larvae hatched by Kawara culture method was compared with the number of eggs counted by Stoll's egg-counting method. It was shown that there was marked positive correlation between the two numbers, and that there was no difference in the range of variation of the numbers contained in one sample between the two methods. This will indicate the accuracy and reliability of Kawara culture method as a means for counting the number of larvae coming out of eggs. The part II concerns with a study in Ancylostoma caninum on the relation between the number and kinds. of parent worms and the development and hatching of eggs. The rate of larvae hatched was determined on the basis of the number of larvae hatched by Kawara culture method and the number of eggs counted by Stoll's egg-counting method. The relations between this rate and the number and kinds of hookworms collected from the sacrificed dogs were studied. The result obtained is summarized as follows: (1) There was no connection whatsoever between the number of parasite worms, the number of parasite female worms and the ratio female/male worms on one hand and the rate of hatching of larvae on the other. (2) There was negative correlation between the logarithm of the number of eggs per day per female and the hatching of larvae. (3) There was negative correlation between the length of the female worm and the rate of hatching of larvae. There seemed to be the same relation between the weight of the female worm and the rate of hatching of larvae. (4) Direct effects of the density on the fluctuations in the rate of hatching of larvae were not clear. It was assumed that the fluctuations might occur as secondary effects of population density. (5) Day to day fluctuations in the rate of hatching of larvae were markedly smaller in the group in which there was smaller number of eggs per gram as counted by Stoll's method. It was disclosed that these fluctuations paralleled day to day fluctuations in the number of eggs counted by Stoll's method.
- 千葉大学の論文
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