Using response surface methodology to optimize process parameters and cross-linking agents for production of combined-cross-linked bovine serum albumin gels(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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概要

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• D-optimal design was employed to optimize the mixture of cross-linking agents formulation: microbial transglutaminase (MTGase) and ribose, and the processing parameters (i.e. incubation and heating time) in the mixture in order to obtain combined-cross-linked bovine serum albumin gels that have high gel strength, pH close to neutral and yet medium in browning. Analysis of variance (ANOVA) showed that the contribution of quadratic term to the model over the linear was significant for pH and L^* value, whereas linear model was significant for gel strength. Optimization study using response surface methodology (RSM) was performed to the mixture components and process variables and the optimum conditions obtained were: MTGase of 1.34-1.43g/100mL, ribose of 1.07-1.16g/100mL, incubation time of 5h at 40℃ and heating time of 3h at 90℃.

著者

• Easa Azhar

  Food Technology Division School Of Industrial Technology University Sains Malaysia

• Gan Chee-Yuen

  Food Technology Division, School of Industrial Technology, University Sains Malaysia

• Alkarkhi Abbas

  Environmental Technology Division, School of Industrial Technology, University Sains Malaysia

• Gan Chee-yuen

  Food Technology Division School Of Industrial Technology University Sains Malaysia

• Alkarkhi Abbas

  Environmental Technology Division School Of Industrial Technology University Sains Malaysia

関連論文

• Using response surface methodology to optimize process parameters and cross-linking agents for production of combined-cross-linked bovine serum albumin gels(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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