Strain improvement of Acremonium cellulolyticus for cellulase production by mutation(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

概要

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In the search for an efficient producer of cellulolytic enzymes, Acremonium cellulolyticus strain C-1 was subjected to mutagenesis using UV-irradiation and N-methyl-N'nitro-N-nitrosoguanidine(NTG) and strain CF-2612 was isolated. Strain CF-2612 exhibited higher filter paperase(FPase) activities(17.8U/ml) than the parent strain C-1(12.3U/ml). Soluble protein production and β-glucosidase activity from strain CF-2612 were also significantly improved. FPase activity, cellulase productivity and yield of CF-2612 using batch culture with 5% Solka Floc in a 2-1 jar fermentor at 30℃ reached 18.0U/ml, 150.0FPU/l/h and 360.0FPU/g carbohydrate, respectively; when fed-batch culture was used with Solka Floc, these values reached 34.6U/ml, 240.3FPU/l/h and 346.0FPU/g carbohydrate, respectively. It was observed that more hydrolyzed glucose was released from pretreated eucalyptus with the enzyme of strain CF-2612, compared with that of the commercial cellulase GC-220. This result was attributed to the higher ratio of β-glucosidase/FPase activity of strain CF-2612. Three distinguishable phases including the periods of primary or second mycelial growth and mycelial fragmentation were proposed in batch culture by A. cellulolyticus.