Carbon tetrachloride-induced hepatotoxicity in pregnant and lactating rats
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概要
- 論文の詳細を見る
Carbon tetrachloride (CCl_4) is well known to induce hepatotoxicity after being metabolized to trichloromethyl free radical (・CCl_3) by CYP2E1. In the present study, the hepatotoxicity induced by a single oral dose (2,000mg/kg) of CCl_4 was compared between pregnant (gestation days (GD) 13 and 19) or postpartum (postpartum days (PPD) 1, 13 and 27) and non-pregnant rats. Hepatotoxicity in CCl_4-treated pregnant rats evaluated by blood chemistry (alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) activities) and histopathological finding (area of damaged hepatocytes) was minimal on GD19, being weaker than that in non-pregnant rats. CYP2E1 expression in non-treated pregnant rats decreased as pregnancy progressed and reached minimum level on GD19. Thus, the degree of CCl_4-induced hepatotoxicity roughly corresponded to CYP2E1 levels during pregnancy. After delivery, hepatotoxicity in CCl_4-treated lactating rats was maximal on PPD13, being stronger than that in non-pregnant rats, and then it decreased slightly on PPD27. The CYP2E1 level in the non-treated lactating rats tended to increase but remained at lower levels until PPD13 compared with that in non-pregnant rats. Thus, the degree of CCl_4-induced hepatotoxicity did not correspond to CYP2E1 levels during lactation. This suggests that during lactation, there may be certain factors other than CYP2E1 expression responsible for the degree of CCl_4-induced hepatotoxicity.
- 日本トキシコロジー学会の論文
- 2009-04-01
著者
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OHISHI Takumi
Toxicology Department, Gotemba Laboratory, Bozo Research Center Inc.
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KITAZAWA Takahiro
Toxicology Department, Gotemba Laboratory, Bozo Research Center Inc.
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NAKAMURA Daichi
Toxicology Department, Gotemba Laboratory, Bozo Research Center Inc.
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NISHIHARA Yoshito
Toxicology Department, Gotemba Laboratory, Bozo Research Center Inc.
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MOCHIZUKI Masahiro
Toxicology Department, Gotemba Laboratory, Bozo Research Center Inc.
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Suzuki Saori
Toxicology Department Gotemba Laboratory Bozo Research Center Inc.
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Ohishi Takumi
Toxicology Dep. Gotemba Lab. Bozo Res. Center Inc.
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Ohishi Takumi
Toxicology Department Gotemba Laboratory Bozo Research Center Inc.
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Nakamura Daichi
Toxicology Department Gotemba Laboratory Bozo Research Center Inc.
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Kamata Takashi
Toxicology Department Gotemba Laboratory Bozo Research Center Inc.
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Kitazawa Takahiro
Toxicology Department Gotemba Laboratory Bozo Research Center Inc.
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Umeshita Kazuhiko
Toxicology Department Gotemba Laboratory Bozo Research Center Inc.
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Nishihata Yoshito
Toxicology Department Gotemba Laboratory Bozo Research Center Inc.
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Mochizuki Masahiro
Toxicology Division Gotemba Laboratory Bozo Research Center Inc.
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Mochizuki Masahiro
Toxicology Department Gotemba Laboratory Bozo Research Center Inc.
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Shimizu Satomi
Toxicology Department Gotemba Laboratory Bozo Research Center Inc.
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Urasoko Yoshinaka
Toxicology Department, Gotemba Laboratory, Bozo Research Center Inc.
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Edamoto Hiroshi
Toxicology Department, Gotemba Laboratory, Bozo Research Center Inc.
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Edamoto Hiroshi
Toxicology Department Gotemba Laboratory Bozo Research Center Inc.
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Mochizuki Masahiro
Gotemba Laboratory Bozo Research Center Inc.
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Nakamura Daichi
Toxicology Dep. Gotemba Lab. Bozo Res. Center Inc.
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Urasoko Yoshinaka
Toxicology Dep. Gotemba Lab. Bozo Res. Center Inc.
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Ohishi Takumi
Gotemba Lab. Bozo Res. Center Inc.
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SUZUKI Saori
Gotemba Laboratory, Bozo Research Center Inc.
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Shimizu Satomi
Gotemba Laboratory Bozo Research Center Inc.
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