リポソームの凍結挙動に関する研究(熱工学,内燃機関,動力など)

概要

論文の詳細を見る
The freezing behavior of liposomes such as internal freezing, contraction by dehydration and disruption of membrane after thawing was examined by microscopic observation. Liposome suspensions were prepared by gentle hydration method using phosphatidylcholine (egg) and distilled water. The observed liposomes ranged in diameter from 5 to 150μm. The sample of liposome suspensions was cooled from -1℃ to -50℃ at cooling rates of 1,2 and 5℃/min and heated to 0℃ at heating rate of 10℃/min. As a result, two different patterns for freezing were observed: internal freezing and contraction without internal freezing. When the internal freezing was observed, the membrane was unexceptionally disrupted after thawing. When the internal freezing was not observed, two different cases were observed after thawing: disruptive and contractive conditions. These different freezing patterns were primarily dependent on the liposome size. In addition, the cooling rate became a key factor determining the freezing patterns in small liposomes.
一般社団法人日本機械学会の論文
2009-02-25