Mutagenesis of longer inserts by the ligation of two PCR fragments amplified with a mutation primer(METHODS)

概要

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We report a method for efficient mutagenesis of DNA in large vectors without subcloning. Two segments of the target DNA sequence, one having a mutation introduced via a mutant primer, were amplified by PCR and then the purified fragments were ligated to a vector. The mutation efficiency was nearly 100%.
社団法人日本生物工学会の論文