Assessment of filter plates for multi-well in-gel digestion of proteins separated by polyacrylamide gel electrophoresis to identify them with LC-ESI/MSMS

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概要

• 論文の詳細を見る

• In-gel digestion is an important technique for proteome analysis using polyacrylamide gel electrophoresis. Although the identification by MSMS ion search is much reliable than PMF, MSMS analysis of peptides separated by nanoLC directly coupled to MS is time consuming and clogging with particles from in-gel digest interrupts separation of peptide by nanoLC. Removal of particles from the digest with filtration is essential for nanoLC analysis, but it is laborious process, when a large number of samples should be processed. Simple and high-throughput method is necessary for in-gel digestion and the sample preparation of nanoLC. The characteristics of filter plates are investigated and a method of in-gel digestion for nanoLC-MSMS systems using inexpensive 96-well filter plate is presented.

• 2005-09-01

著者

• KAWASAKI Hiroshi

  Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University

• Nanri Tomohiro

  Supra Molecular Biology International Graduate School Of Arts And Sciences Yokohama City University

• Yamanaka Yuko

  Supra Molecular Biology International Graduate School Of Arts And Sciences Yokohama City University

• 亀山 一央

  日本ミリポア

• Kameyama Kazuhisa

  Department Of Chemistry Faculty Of Science And Technology Sophia University

• Kameyama Kazuhisa

  Nihon-millipore K.k.

• ARIMA Mikiko

  Supra Molecular Biology, International Graduate School of Arts and Sciences, Yokohama City Universit

• Arima Mikiko

  Supra Molecular Biology International Graduate School Of Arts And Sciences Yokohama City University

• Kawasaki Hiroshi

  Supramolecular Biology International Graduate School Of Arts And Sciences Yokohama City University

• Kawasaki Hiroshi

  Supra Molecular Biology International Graduate School Of Arts And Sciences Yokohama City University

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