Hepatitis B virus-positive blood donors in the early stages of Hepatitis B virus infection : Significance of nucleic acid amplification testing

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概要

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• Since 1999, 1,387,094 units of serologically negative blood donations to the Hyogo Blood Center have been screened by nucleic acid amplification testing (NAT). Thirty hepatitis B virus (HBV)-DNA positive donors were identified from among all the donors. Twenty-one out of the 30 selected donors were contacted and monitored with their courtesy and consent. Their HBV serological markers, HBV copy number, and alanine transaminase (ALT) levels were analyzed. They were then classified into three groups according to the pattern of increase in their ALT levels. Nine cases in Group I showed more than 1,000 IU/L ALT and 9 cases in Group II showed slightly higher levels. Because the monitoring started during the very early stage of the serological negative period, all the phenomena associated with the development of typical hepatitis symptoms became evident in Groups I and II. On the other hand, the ALT levels of three cases in Group III remained within the normal range or only slightly increased, and these three were asymptomatic for hepatitis B. In two cases, a single nucleotide substitution, G1896A, was observed in the precore region but not in the core promoter region; this substitution result in the tryptophan codon (TGG) being converted to a stop codon (TAG). Another case showed a mixed type mutation involving the wild type and G1896A substitution. Although many reports on fulminant or subfulminant hepatitis patients associated with precore-defective HBV mutants have been published, asymptomatic hepatitis B patient with precore-defective HBV mutants have not been reported. Thus, it is suggested that a mutation in the core promoter (T1754C/G and/or A1762T/G1764A) might cause an increase in the viral growth rate or suppression for viral increase.

• 神戸大学の論文

著者

• Imoto Shion

  Laboratory Of Hyogo Red Cross Blood Center

• Nishiyama Kaoru

  Department of Medical Technology Faculty of Health Sciences, Kobe University School of Medicine

• Mito Hisashi

  Laboratory Of Hyogo Red Cross Blood Center

• Nishiyama Kaoru

  Department Of Medical Technology Faculty Of Health Sciences Kobe University School Of Medicine

• Yamashita Mariko

  Laboratory of Hyogo Red Cross Blood Center

• Yamashita Mariko

  Laboratory Of Hyogo Red Cross Blood Center:department Of Medical Technology Faculty Of Health Scienc

• Yamashita Mariko

  Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Shizuoka University

• Nishiyama Kaoru

  Department of Medical Technology, Faculty of Health Sciences, Kobe University School of Medicine

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