Improving Enzyme Immobilization in Mesocellular Siliceous Foams by Microwave Irradiation(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
- 論文の詳細を見る
Microwave irradiation was used to immobilize papain and penicillin acylase in mesocellular siliceous foams (MCFs) at low temperature. The maximum loading of papain reached 984.1mg/g, 1.26 times that obtained using the conventional, non-microwave-assisted method. The half-life (t_<0.5>) of papain immobilized in MCFs by microwave irradiation at 80℃ was 17h, 5.21 times that of papain immobilized by conventional means. The activities of papain and penicillin acylase immobilized with the microwave-assisted method were 779.6U/mg and 141.8U/mg respectively, 1.86 and 1.39 times of those obtained without microwave immobilization. Using microwave irradiation it only took 140s for penicillin acylase, an enzyme of large dimensions, to be immobilized in MCFs. In contrast, it took 15h to do the same using the conventional method. The results showed that microwave irradiation improved the adsorption and immobilization of enzymes in mesocellular siliceous foams.
- 公益社団法人日本生物工学会の論文
- 2008-09-25
著者
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Ouyang Pingkai
National Engineering Research Center For Biotechnology:college Of Life Science And Pharmaceutical En
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Wang Hua
National Laboratory Of Plant Molecular Genetics Shanghai Institute Of Plant Physiology And Ecology S
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Zhou Cheng
College Of Biotechnology And Pharmaceutical Engineering Nanjing University Of Technology
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ZHU Shemin
College of Materials Science and Engineering, Nanjing University of Technology
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SHEN Shubao
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology
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DU Zhiqiang
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology
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WANG Anming
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology
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Wang Anming
National Engineering Research Center for Biotechnology
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Zhou Cheng
National Engineering Research Center for Biotechnology
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Du Zhiqiang
National Engineering Research Center for Biotechnology
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Liu Mingqing
National Engineering Research Center for Biotechnology
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Shen Shubao
National Engineering Research Center for Biotechnology
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Zhu Shemin
State Key Lab. Of Materials-oriented Chemical Engineering Coll. Of Materials Sci. And Engineering Na
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Shen Shubao
State Key Lab. Of Materials-oriented Chemical Engineering Coll. Of Materials Sci. And Engineering Na
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Du Zhiqiang
College Of Biotechnology And Pharmaceutical Engineering Nanjing University Of Technology
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Liu Mingqing
National Engineering Research Center For Biotechnology:college Of Life Science And Pharmaceutical En
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Wang Hua
National Engineering Research Center For Biotechnology:college Of Life Science And Pharmaceutical En
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Wang Hua
National Engineering Research Center For Biotechnology
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- Improving Enzyme Immobilization in Mesocellular Siliceous Foams by Microwave Irradiation