Improved Method for Immobilization of Trypsin to Enhance Catalytic Performance of Trypsin in Confined Nanospaces
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概要
- 論文の詳細を見る
Mesocellular siliceous foams (MCFs) functionalized with different groups were used in the trypsin immobilization process. Macromolecular reagents were co-assembled with trypsin in the MCFs to create a microenvironment resembling that in living cells. The catalytic characteristics and stability of trypsin preparations were investigated. The relative activity of immobilized trypsin was 122.9% when the enzyme was immobilized adsorptively in the MCFs. Trypsin assembled with BSA in the MCFs attained maximum specific activity, 1.33 times that of the unmodified immobilized trypsin. The Km value of the BSA derivative of trypsin was reduced to 15.9% and 16.6% relative to the valve for native and solely immobilized trypsin, respectively, while the Kcat/Km value of trypsin assembled with BSA was enhanced to 148%. The residual activity of trypsin assembled with BSA was 48.5% and 29.4% at 50°C and 60°C, respectively, after incubation for 60 min.
- 社団法人 化学工学会の論文
- 2010-07-01
著者
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Zhou Cheng
College Of Biotechnology And Pharmaceutical Engineering Nanjing University Of Technology
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Cen Tao
College Of Biotechnology And Pharmaceutical Engineering Nanjing University Of Technology
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Jiang Bo
College Of Biotechnology And Pharmaceutical Engineering Nanjing University Of Technology
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WU Xiuming
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology
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ZHU Shemin
College of Materials Science and Engineering, Nanjing University of Technology
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SHEN Shubao
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology
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Zhu Shemin
State Key Lab. Of Materials-oriented Chemical Engineering Coll. Of Materials Sci. And Engineering Na
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Wu Xiuming
College Of Biotechnology And Pharmaceutical Engineering Nanjing University Of Technology
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Shen Shubao
State Key Lab. Of Materials-oriented Chemical Engineering Coll. Of Materials Sci. And Engineering Na
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