Chromosome Fragments have the Potential to Predict Hyperthermia-induced Radio-sensitization in Two Different Human Tumor Cell Lines

スポンサーリンク

概要

• 論文の詳細を見る

• Cellular radiosensitivity, assessed by loss of clonogenecity, has been shown to correlate with the number of radiation-induced chromosomal aberrations. Also an increased radiosensitivity by hyperthermia has been shown to correlate with an increase in chromosomal aberrations. Therefore, determination of the number of chromosomal aberrations might be used as an assay to predict the radiosensitivity of tumors pretreated with hyperthermia at clinically relevant temperatures. The use of premature chromosome condensation combined with fluorescent in situ hybridisation (PCC-FISH) has been shown to be clinically applicable. Therefore, the use of chromosomal aberrations as determined with PCC-FISH for the prediction of hyperthermia-induced radio-sensitization in human tumor cells was investigated. Confluent cultures of SW-1573 (human lung carcinoma) and RKO (human colorectal carcinoma) cells were treated with 1h 41℃ or 43℃ hyperthermia prior to γ-irradiation. Clonogenic cell survival and induction of chromosomal aberrations (unrejoined chromosomal fragments and translocations), by PCC-FISH, were studied at 24h after treatment. Pre-treatment with hyperthermia at 41℃ for 1h enhanced the radiosensitivity of RKO cells but not of SW-1573 cells. Increasing the temperature to 43℃ for 1h enhanced the radiosensivity of SW-1573 cells. When radio-sensitization was observed, a significant increase in the number of unrejoined chromosomal fragments was found but the frequency of translocations was not increased. Hyperthermia-induced radio-sensitization is correlated with an increase in unrejoined chromosomal fragments. This suggests that determination of the number of chromosomal fragments after hyperthermia and radiation treatment might be used for the prediction of combined treatment response in cancer patients.

• 日本放射線影響学会の論文
• 2008-09-16

著者

• Bree Chris

  Academic Medical Center University Of Amsterdam Laboratory For Experimental Oncology And Radiobiolog

• Haveman J

  Academic Medical Center University Of Amsterdam Laboratory For Experimental Oncology And Radiobiolog

• Haveman Jaap

  Academic Medical Centre University Of Amsterdam Laboratory For Experimental Oncology And Radiobiolog

• Franken Nicolaas

  Academic Medical Center University Of Amsterdam Laboratory For Experimental Oncology And Radiobiolog

• Cate Rosemarie

  Academic Medical Center University Of Amsterdam Laboratory For Experimental Oncology And Radiobiolog

• BERGS Judith

  Academic Medical Center, University of Amsterdam, Laboratory for Experimental Oncology and Radiobiol

• MEDEMA Jan

  Academic Medical Center, University of Amsterdam, Laboratory for Experimental Oncology and Radiobiol

• Medema Jan

  Academic Medical Center University Of Amsterdam Laboratory For Experimental Oncology And Radiobiolog

• Bergs Judith

  Academic Medical Center University Of Amsterdam Laboratory For Experimental Oncology And Radiobiolog

• Haveman Jaap

  Academic Medical Center, University of Amsterdam, Laboratory for Experimental Oncology and Radiobiology (LEXOR), Center for Experimental Molecular Medicine, Department of Radiation Oncology

関連論文

• Radiosensitization by Bromodeoxyuridine and Hyperthermia: Analysis of Linear and Quadratic Parameters of Radiation Survival Curves of Two Human Tumor Cell Lines
• Residual Late Radiation Damage in Mouse Stromal Tissue Assessed by the Tumor Bed Effect
• Chromosome Fragments have the Potential to Predict Hyperthermia-induced Radio-sensitization in Two Different Human Tumor Cell Lines

スポンサーリンク