Effect of Extract of Pomegranate (Punica granatum L.) on Glycated Protein-iron Chelate-induced Toxicity : An In Vitro Study on Human Umbilical-vein Endothelial Cells

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概要

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• The binding of advanced glycation endproducts (AGEs) to a cell-surface receptor for AGEs (RAGE) induces the formation of reactive oxygen species (ROS), which have been causally implicated in the pathogenesis of diabetic vascular complications. Pomegranate fruit extract (PFE) contains, a naturally occurring polyphenolic compound reported to possess potent radical-scavenging and antioxidant properties and to display significant cardiovascular protective action. In this study, we investigated whether PFE could inhibit glycated protein-iron chelate-induced toxicity by interfering with ROS generation in human umbilical-vein endothelial cells (HUVEC). Glycated fetal bovine serum (GFBS) was prepared by incubating fetal bovine serum (FBS) with high-concentration glucose. Stimulation of cultured HUVECs with 50 mM 1 ml of GFBS significantly enhanced lipid peroxidation and decreased antioxidant enzyme activities and levels of phase II enzymes. However, preincubation of the cultures with PFE resulted in a marked decrease in the level of lipid peroxide (LPO) and increase in the levels of antioxidant enzymes in a PFE concentration-dependent manner. These results demonstrate that PFE could inhibit LPO and enhance the antioxidant enzyme status in GFBS-iron chelate exposed endothelial cells by suppressing ROS generation, thereby limiting the effects of the AGE-RAGE interaction. Hence, PFE may have therapeutic potential in the prevention and treatment of vascular complications in diabetic patients.

• 公益社団法人日本薬学会の論文
• 2008-08-01

著者

• SAKTHISEKARAN Dhanapal

  Department of Medical Biochemistry, University of Madras

• Rajendran Peramaiyan

  Department Of Medical Biochemistry Dr. Alm Pg Institute Of Basic Medical Sciences University Of Madr

• Nishigaki Ikuo

  NPO International Laboratory of Biochemistry

• Rajendran Peramaiyan

  NPO International Laboratory of Biochemistry

• Venugopal Ramachandran

  NPO International Laboratory of Biochemistry

• Ekambaram Ganapathy

  NPO International Laboratory of Biochemistry

• Nishigaki Yutaka

  Tokyo Metropolitan Institute of Gerontology

• Sakthisekaran Dhanapal

  Department Of Medical Biochemistry Dr. Alm Pg Institute Of Basic Medical Sciences University Of Madr

• Sakthisekaran Dhanapal

  Department Of Medical Biochemistry Alm Postgraduate Institute Of Basic Medical Sciences University O

• Ekambaram Ganapathy

  Department Of Medical Biochemistry Dr. Alm Pg Institute Of Basic Medical Sciences University Of Madr

関連論文

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• Effect of Extract of Pomegranate (Punica granatum L.) on Glycated Protein-iron Chelate-induced Toxicity : An In Vitro Study on Human Umbilical-vein Endothelial Cells
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