Stable Repeated-Batch Production of Recombinant Dye-Decolorizing Peroxidase(rDyP) from Aspergillus oryzae(ENVIRONMENTAL BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
Recombinant Aspergillus oryzae expressing a dye-decolorizing peroxidase gene(dyp) was cultivated for repeated-batch production of recombinant dye-decolorizing peroxidase(rDyP) using maltose as a carbon source. High-level rDyP activity in limitation of carbon and nitrogen sources was maintained stably for 26 cycles of repeated 1-d batches of A. oryzae pellets without any additional pH control. Cultures maintained at 4℃ for 20 d resumed rDyP production following a single day of incubation. One liter filtrated crude rDyP containing 4600 U rDyP decolorized 5.07 g RBBR at the apparent decolorization rate of 17.7 mgl^<-1> min^<-1>.
- 2008-06-25
著者
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SHODA Makoto
Chemical Resources Laboratory, Tokyo Institute of Technology
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Shoda Makoto
Chemical Resources Laboratory Tokyo Institute Of Technology
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Shoda Makoto
R1-30 Chemical Resources Laboratory Tokyo Institute Of Technology
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Sugano Yasushi
R1-30 Chemical Resources Laboratory Tokyo Institute Of Technology
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Sugano Yasushi
Chemical Resources Laboratory Tokyo Institute Of Technology
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Shakeri Mozaffar
Chemical Resources Laboratory Tokyo Institute Of Technology
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