Characterization of Aryl Alcohol Oxidase Produced by Dye-Decolorizing Fungus, Geotrichum candidum Dec1
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概要
- 論文の詳細を見る
Aryl alcohol oxidase (AAO) produced by Geotrichum candidum Dec1 (Dec1), a newly isolated decolorizing fungus, was purified by ultrafiltration and by using diethylaminoethyl (DEAE) Sephacel, Butyl-Toyopearl and Mono-Q columns. H_2O_2 produced by concomitant AAO oxidation of veratryl alcohol (VA) to veratraldehyde was consumed by a peroxidase (DyP) purified from Dec1 culture, leading to the decolorization of a dye in vitro. In the liquid culture of Dec1, the existence of H_2O_2 and veratraldehyde was confirmed during cultivation, when dye-decolorization and AAO activities were maintained. This indicates that VA produced by Dec1 was oxidized by AAO to veratraldehyde, generating H_2O_2, which supported dye-decolorizing activity of Decl in vivo. The prevention of polymerization of DyP oxidation products of a dye in the presence of AAO was shown.
- 社団法人日本生物工学会の論文
- 2001-02-25
著者
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SHODA Makoto
Chemical Resources Laboratory, Tokyo Institute of Technology
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UEMATSU Yoshiko
Department of Applied Chemistry, Tokyo Institute of Polytechnics
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Shoda Makoto
Chemical Resources Laboratory Tokyo Institute Of Technology
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Uematsu Yoshiko
Department Of Applied Chemistry Tokyo Institute Of Polytechnics
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Uematsu Yoshiko
Department Of Industrial Chemistry Tokyo Institute Of Polytechnics
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KIM SEONG
Chemical Resources Laboratory, Tokyo Institute of Technology
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SUZUKI NORIKUNI
Department of Industrial Chemistry, Tokyo Institute of Polytechnics
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Kim Seong
Chemical Resources Laboratory Tokyo Institute Of Technology
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Suzuki N
Department Of Industrial Chemistry Tokyo Institute Of Polytechnics
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