Enhancement of Nuclear Localization of Metallothionein by Nitric Oxide

概要

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Nitric oxide (NO) enhanced the nuclear localization of metallothionein (MT) in digitoninpermeabilized semi-intact HeLa cells. Although 1-Hydroxy-2-oxo-3-(3-aminopropyl)-3-isopropyl-1-triazene (NOC5), an NO donor, enhanced the nuclear localization of MT in a manner similar to hydrogen peroxide (H_2O_2), a tenfold higher concentration of NOC5 was required to achieve the same effect as H_2O_2, suggesting that an endogenous NO scavenger existed in the reaction mixture of the nuclear import assay system that we used. We also evaluated the effect of NO on the nuclear localization of MT in intact HeLa cells. Treatment with NOCS induced the nuclear localization of MT pre-induced with zinc, and its effect was greater than that of H_2O_2. The induced MT was localized in the nucleus and the cytoplasm. The results suggest that MT can scavenge NO using the sulfhydryl groups of cysteines in its molecule to form nitrosothiol, thereby reducing nuclear and cytoplasmic damage by NO.
公益社団法人日本薬学会の論文
2008-06-01