16 放線菌の気菌糸を誘導するlantibiotic様ペプチド類の構造決定(口頭発表の部)

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概要

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• Streptomycetes are gram-positive soil bacteria which have complex life cycle similar to that of fungi. Besides, whole genome sequences of several strains were completed recently. On the basis of these backgrounds, S. coelicolor is a model organism for the study of morphological changing system. When it is cultured on an agar medium, it makes substrate mycelium. After 2-3 days, the generation of aerial hyphae starts from substrate mycelium into the air. Finally, spores are produced from aerial hyphae through metamorphose. It was known that the signal-cascade system via several signal molecules involved in the aerial hyphae formation of S. coelicolor. The final product of the cascade was known as SapB (Spore Associate Protein B) which induces aerial hyphae directly. We recently found that SapB was a lantibiotic-like peptide derived from the product of the developmental gene ramS. The structure was deduced as 1 by N-terminus sequencing after reduction and tandem TOF-mass. SapB lacked of the antimicrobial activity despite the fact that it had a lantibiotic-like structure. This was the first example of a morphogenetic role for an antibiotic molecule. To figure out the diversity of lantibiotic like peptide in streptomycetes, we isolated another peptide SapT from S. tendae. The structure of SapT was elucidated as 2 by analyzing the data of NMR and TOF-MS spectra. Although SapT has different structure from that of SapB, it showed a similar inducing activity of aerial formation to S. coelicolor. Surprisingly, hydrophobin, an aerial hyphae inducing protein of fungi, also induced aerial formation of S. coelicolor, although hydrophobin is a glycosylated protein which does not have any structure similarity to SapT and SapB. It was indicated by Wosten et al. that hydrophobin had amphiphatic structure and cause surfactant effect on surface of agar plate. Lowering surface tension by the surfactant activity helped to escape substrate mycelium into the air. We applied 3D molecular modeling method to SapB and SapT. The result indicated that the peptides had amphipatic structures and induced aerial hyphae in the same manner with hydrophobin.

• 2006-09-15

著者

• 小谷 真也

  静大院・創造:静大農・応生化

• 小谷 真也

  食品総合研究所

• Willey Joanne

  ホフストラ大学

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• 16 放線菌の気菌糸を誘導するlantibiotic様ペプチド類の構造決定(口頭発表の部)

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