15 人工基貭からのジベレリン関連化合物の微生物生産

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Many researchers focused their attention to the microbial productions of biological active compounds and their analogues from artificial substrates. This paper will report the microbial productions of gibberellins and related compounds from artificial substrates by G. fujikuroi. At first, the substrate specificity of the enzymes operative in the gibberellin biosynthesis was examined and kaurene analogues, (1), (4), (5) and (6) were found to be converted to gibberellin-like compounds by mycelium suspension of G. fujikuroi. Two kaurene analogues, (1)(200 mg) and (4)(500 mg) were then converted to GA-like compounds in the mycelium suspension, in which endogenous biosynthesis of gibberellins was blocked by CCC. The acidic metabolites from (1) were p-bromophenacylated and separated by PTLC and LC. The purified phenacyl esters were dephenacylated and methylated to afford methyl esters of GA-like compounds, A-I and A-II. The alcohol(4) was similarly converted to six GA-like compounds, B-I to B-V and a kaurenoid, B-VI. The acid(7)(600 mg) gave three GA-like compounds, C-I, II, andIII, in the mycelium suspension of G. fujikuroi containing 1-n-decylimidazole. Microbial production of gibberellin-like compounds is now in progress in large scale for tests of their biological activities.
天然有機化合物討論会の論文
1979-09-20