55 Aspergillus terreusの代謝産物の生合成 : Pulvinone誘導体の酵素によるプレニル化

概要

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The 33-55% ammonium sulfate fraction of a cell-free extract from Aspergillus terreus catalyzed the transfer of the C_5 units from dimethyl-allyl pyrophosphate to both of the aryl rings of dihydroxypulvinone (Compd E, 5), giving a bis-prenyl derivative (9), a possible precursor for the metabolites, Compd A (1) and Compd B (2). A tris-prenyl derivative of Compd E was also found as the minor product in the enzymatic reaction between Compd E and dimethylallyl pyrophosphate. Trihydroxypulvinone (Compd G, 7) was also accepted as the substrate for this enzyme, reacting with two molecules of dimethylallyl pyrophosphate to give a prenylated derivative (12) which is a possible precursor for Compd C (3), Compd D (4) and Compd F (6). The enzyme was stable at least for a month when stored frozen at -20°,and could be partially purified.
天然有機化合物討論会の論文
1975-10-01