A Pectic Polysaccharide Isolated from the Roots of Bupleurum falcatum L. Stimulates the Tyrosine Phosphorylation of Lipid Rafts of Murine B Cells(Highlighted paper selected by Editor-in-chief,Pharmacognosy)
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概要
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Bupleuran 2IIc, a pectic polysaccharide isolated from the roots of Bupleurum falcatum L., was previously characterized as a T cell-independent B cell mitogen. The endo-(1→4)-α-D-polygalacturonase-resistant moiety of bupleuran 2IIc (bupleuran 2IIc/PG-1) was the active site for expression of the activity, and expression of the cyclin D2 gene by bupleuran 2IIc/PG-1 may be mediated via activation of Src family tyrosine kinase, phosphatidylinositol 3-kinase (PI 3-K) and phospholipase C (PLC)-γ followed by activation of protein kinase C (PKC) and calcium mobilization (Matsumoto et al., Int. Immunopharmacol., 5, 1373-1386 (2005)). Plasma membrane microdomains (lipid rafts) are enriched in signaling molecules and suggested to be involved in numerous cell functions, including membrane traffic and signaling. When B cells were stimulated with bupleuran 2IIc/PG-1, clustering of membrane lipid rafts was observed. To consider whether lipid rafts are implicated in bupleuran 2IIc/PG-1-mediated B cell proliferation, we analyzed the phosphorylation of tyrosine residues of proteins in lipid rafts. When murine B cells were stimulated with bupleuran 2IIc/PG-1, tyrosine phosphorylation of proteins in lipid rafts fraction was observed within 5min. Tyrosine phosphorylation in lipid rafts fraction by bupleuran 2IIc/PG-1 was inhibited by the Src-family tyrosine kinase inhibitor, PP2. Together with previously published data, the results presented in this study suggest that activation of signaling molecules in lipid rafts by stimulation of bupleuran 2IIc/PG-1 contributes to B cell proliferation as the membrane-proximal signaling event.
- 公益社団法人日本薬学会の論文
- 2008-05-01
著者
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YAMADA Haruki
The Kitasato Institute
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Matsumoto Tsukasa
The Kitasato Institute For Life Sciences And Graduate School Of Infection Control Sciences Kitasato
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Matsumoto Tsukasa
The Kitasato Institute For Life Sciences And Graduate School Of Infection Control Sciences Kitasato
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Yamada Haruki
The Kitasato Institute For Life Sciences And Graduate School Of Infection Control Sciences Kitasato
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HOSONO-NISHIYAMA Kanako
The Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato
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Hosono-nishiyama Kanako
The Kitasato Institute For Life Sciences And Graduate School Of Infection Control Sciences Kitasato
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- A Pectic Polysaccharide Isolated from the Roots of Bupleurum falcatum L. Stimulates the Tyrosine Phosphorylation of Lipid Rafts of Murine B Cells(Highlighted paper selected by Editor-in-chief,Pharmacognosy)