Galectin-1はヒト口腔扁平上皮癌細胞のアノイキスを抑制する

概要

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In order to determine the factors that relate to anoikis of the human oral squamous cell carcinoma (hOSCC) cells, we tried to search the proteins using the HSC-3 cell with metastaticity and the HSC-2 cells without metastaticity. The HSC cells were cultured in a dish coated with poly-HEMA to prevent cell adhesion, and then the degree of cell death was examined. The ratio of cell death for HSC-2 cells was significantly higher than that for HSC-3 cells, and the result of TUNEL staining showed that the cell death was apoptosis. The level of anoikis in HSC-2 cells was notably higher than that in HSC-3 cells. The expression level of TrkB, caveolin-1, and galectin-3 genes that are known as factors related to anoikis was investigated by RT-PCR. There was no significant difference in the gene expression between HSC-2 and HSC-3 cells. Galectin-1 was found by the proteome analysis and the protein was expressed more strongly in the HSC-3 cells as compared with the HSC-2 cells. A similar result was obtained in the amount of the mRNA expression. The anoikis of HSC-3 cells was caused strongly by the addition of lactose that inhibits the binding of galectin-1 to the cell membrane. When recombinant-galectin-1 was added to the medium, the level of anoikis in HSC-2 cells was decreased significantly. From these results, it was suggested that galectin-1 is the factor that suppresses anoikis in hOSCC cells.
岩手医科大学歯学会の論文
2007-12-20