Reversible Alterations in Morphology and Proliferation of Mouse H-1 (ES) Cells in DME Versus L15F10 Medium
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概要
- 論文の詳細を見る
The morphology and proliferation of mouse H-1 (ES) cells were examined in Dulbecco's modified Eagle's (DME) and Leibovitz's L15 : Ham's F10 (7 : 3) mixture (L15F10) medium. In the presence of sufficient concentrations of leukemia inhibitory factor (LIF), the H-1 cells proliferated with a doubling time less than 22 h and formed round colonies of tightly packed cells both in the DME and L15F10 media. In the absence of LIF, the H-1 cells could proliferate in L15F10, but not in DME. The H-1 cells that had been in L15F10 medium showed similar morphology and growth responses as those in DME medium when they were returned to DME medium. It was concluded that the morphology and cell cycle parameters (durations of G_1, S and G_2+M phases) of H-1 cells are altered depending on the medium used.
- 金沢医科大学の論文
著者
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YAMAGISHI Hiroko
Division of Basic Science, Research Institute of Medical Science, Kanazawa Medical University
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MIYAGOSHI Minoru
Division of Basic Science, Research Institute of Medical Science, Kanazawa Medical University
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Fujikawa-yamamoto Kohzaburo
Division Of Basic Science Research Institute Of Medical Science Kanazawa Medical University
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Fujikawa-yamamoto Kohzaburo
Division Of Cell Medicine Research Institute Of Medical Science Kanazawa Medical University
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Miyagoshi Minoru
Division Of Cell Medicine Research Institute Of Medical Science Kanazawa Medical University
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Yamagishi Hiroko
Division Of Basic Science Research Institute Of Medical Science Kanazawa Medical University
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- Reversible Alterations in Morphology and Proliferation of Mouse H-1 (ES) Cells in DME Versus L15F10 Medium
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