Construction of plasmids for gene disruption of two chromosomal rrn operons in Haloarcula japonica strain TR-1

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概要

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• Two plasmids derived from halophile-specific plasmid pWL102 with upstream and downstream region of rrn operons, were constructed, in order to disrupt rrn operons, rrnA and rrnB on chromosomes in extremely halophilic archaeon Haloarcula japonica strain TR-1. One of these plasmids, pDT2, for disruption of rrnA operon, is derived from DNA fragments of upstream (676 bp) downstream region (1, 072 bp) linked with the 27 bp of synthetic linker. Second plasmid, pDT3, for disruption of rrnB operon, is also derived from DNA fragments of upstream (668 bp) downstream region (597 bp) linked with the 27 by of synthetic linker. Gene disruption of rrn operons on the chromosomes in extremely halophilic archaeon H. japonica strain TR-1, using these plasmids with homologous recombination, is definitely not only essential, but also useful, to obtain mutants disrupted in the genes of rrn operons.

• 近畿大学の論文
• 2006-12-20

著者

• 仲宗 根薫

  近畿大学工学部生物化学工学科

• 前田 豊

  近畿大学工学部生物化学工学科

• 冨永 大輔

  近畿大学工学部生物化学工学科

• 植木 俊一

  近畿大学工学部生物化学工学科

• 前田 豊

  近畿大学大学院システム工学研究科

関連論文

• Construction of plasmids for gene disruption of two chromosomal rrn operons in Haloarcula japonica strain TR-1
• Isolation and characterization of the genes for RNA polymerase subunits from Haloarcula japonica strain TR-1
• Isolation and characterization of three protease-producing bacteria from a deep-sea mud of Japan Sea
• Construction and genetic analyses of mutant for rrnB operon in extremely halophilic archaeon, Haloarcula japonica
• Structural analysis of S10-spc operons encoding genes for ribosomal proteins in Haloarcula japonica strain TR-1

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