NMR METABOLIC PROFILING COMBINED WITH TWO-STEP PRINCIPAL COMPONENT ANALYSIS FOR TOXIN-INDUCED DIABETES MODEL RAT USING URINE

スポンサーリンク

概要

• 論文の詳細を見る

• Nuclear magnetic resonance-based metabolic profiling (NMR-MP) was applied to evaluate disorder model animals using urine. Diabetic nephropathy was established in this experiment by administering streptozotocin to Wistar rats, which immediately developed diabetes after toxin-treatment and then gradually produced albumin-containing urine (albuminuria). Urine samples were collected for the first 4 weeks after toxin treatment. Predominant urinary sugar signals were seen in ^1H-NMR spectra of diabetes rat urine, and spectra were processed and subjected to multivariate analysis. Principal component analysis (PCA) identified 3 outliers among 20 individuals. Outlier rats did not develop urinary sugar and were later found to be rats insufficient to establish diabetes models. A second PCA was performed excluding the additional glucose-signal region (3.2-6.3ppm), as glucose signals had a predominant effect that may mask details of other metabolic profiles. Consequently another outlier was revealed. This exceptional rat did not develop albuminuria even after producing glucosuria for 14 weeks. NMR metabolic profiling provides good guidance to evaluate biophysical conditions of animals, enabling detection of abnormalities in the early stage of toxicological experiments.

• 日本トキシコロジー学会の論文
• 2007-10-15

著者

• NEMOTO Tadashi

  Biomedicinal Information Research Center, AIST

• Ando Itiro

  Planning For Drug Development And Clinical Evaluation Tohoku University Graduate School Of Pharmaceu

• Nemoto Tadashi

  National Institute Of Bioscience And Human-technology

• Fujiwara Masako

  Planning For Drug Development And Clinical Evaluation Tohoku University Graduate School Of Pharmaceu

• KANAZAWA Kenji

  National Institute of Bioscience and Human-Technology, AIST

• Kanazawa Kenji

  Biomedicinal Information Res. Center (birc) National Inst. Of Advance Industrial Sci. And Technol. (

• Natori Yasuhiro

  International Medical Center Of Japan

• ANDO Itiro

  National Institute of Advance Industrial Science and Technology (AIST)

• KATAOKA Taeko

  National Institute of Advance Industrial Science and Technology (AIST)

• ARIFUKU Kazunori

  JEOL DATUM LTD.

• FUJIWARA Masako

  Century COE Program, Graduate School of Pharmacy, Tohoku University

• FUJIWARA Masako

  Division of Molecular Metabolism and Diabetes, Graduate School of Medicine, Tohoku University

• KANAZAWA Kenji

  National Institute of Advance Industrial Science and Technology (AIST)

• Arifuku Kazunori

  JEOL Co., Ltd.

関連論文

• Quantification of molecules in 1H-NMR metabolomics with formate as a concentration standard
• Quantification of molecules in ^1H-NMR metabolomics with formate as a concentration standard
• 1P345 NMRメタボロミクスの医療への応用 : 透析患者血漿と廃液の解析(その他,第48回日本生物物理学会年会)
• Structural Clarification of Caulerpa Cell Wall β-1, 3-Xylan by NMR Spectroscopy
• Structures of Caulerpa Cell Wall Microfibril Xylan with Detection of β-1,3-Xylooligosac-charides as Revealed by Matrix-assisted Laser Desorption Ionization/Time of Flight/Mass Spectrometry
• Developmental delineation of metabolic-patterns of neonatal mice by using NMR-metabolic profiling on highly-diluted urine
• Studies on Ring Region of Human Collagen α2(IV) Recognized By Monoclonal Antibodies
• Investigation of Low Cycle Fatigue at Elevated Temperatures : Dependence of Behavior of Fatigue Cracks on the Conditions of Temperature and Plastic Strain Range
• Structural studies of Endothelin peptides : ET-2
• Structure Analyses of Novel Peptides of Endothelin Family (3) : Steric Structure Analysis of Endothelin-2 by NMR Spectroscopy
• NMR METABOLIC PROFILING COMBINED WITH TWO-STEP PRINCIPAL COMPONENT ANALYSIS FOR TOXIN-INDUCED DIABETES MODEL RAT USING URINE
• Multivariate Analysis for ^1H-NMR Spectra of Two Hundred Kinds of Tea in the World
• Pattern Recognition Analysis for Classification of Hypertensive Model Rats and Diurnal Variation Using ^1H-NMR Spectroscopy of Urine
• On the Formation of Striation on the High-Temperature Low-Cycle Fatigue Fracture Surface of Type 316 Austenitic Stainless Steel
• Biotin Ameliorates Muscle Cramps of Hemodialysis Patients : A Prospective Trial
• Absolute Quantitation of Stevioside and Rebaudioside A in Commercial Standards by Quantitative NMR

もっと見る 閉じる

スポンサーリンク