Effect of Heat Treatment on Catabolites Formation in Relation to Chlorophyll Degradation during Storage of Broccoli (Brassica olearacea L. Italica Group) Florets
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概要
- 論文の詳細を見る
The effects of heat treatment on the formation of chlorophyll (Chl) derivatives and Chl-degrading enzyme activities in stored broccoli (Brassica oleracea L. Italica Group 'Ryokutei') florets were determined. The Chl a level barely changed during heat treatment, but each Chl a derivative level changed. Chlorophyllide (Chlide) a, pheophorbide (Pheide) a, C13^2-hydroxychlorophyll (C13_2-OHChl) a, Chl a', an isomer of Chl a, and pheophytin (Phy) a were detected as a Chl derivative during heat treatment and during storage after treatment. Chlide a, Pheide a, and C13^2-OHChl a levels decreased during 2-h heat treatment (50℃), whereas Chl a' and Phy a levels increased. Chl-degrading enzyme activities, in particular, Mg-dechelation activity, were effectively suppressed after 2h of heat treatment. The content of Chls a and b in control broccoli florets decreased greatly during storage at 15℃, while the content in heat-treated broccoli florets hardly changed at all. In Chl derivatives, the Pheide a level in broccoli florets treated with or without heat treatment, especially the former, increased appreciably during storage. The Chlide a level in control florets decreased markedly during storage. On the other hand, the Chlide a level in heat-treated broccoli florets did not change during storage. Mg-dechelation activity in control florets markedly increased after 4 days of storage at 15℃, but the enhancement of the activity was suppressed by heat treatment. These findings suggest that Chl derivatives, especially Pheide a, are accumulated as intermediates in heat-treated broccoli florets, and Mg-dechelating action, in conjunction with that of chlorophyllase and Chl-degrading peroxidase, could be involved in Chl degradation in stored broccoli florets.
- 園芸学会の論文
著者
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Mori Tomohiko
Faculty Of Agriculture Yamaguchi University:(present)kobeya Bakery Company
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Kanlayanarat Sirichai
Division Of Postharvest Technology School Of Bioresources And Technology King Mongkut's Univers
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Kanlayanarat Sirichai
Division Of Postharvest Technology King Mongkut's University Of Technology Thonburi
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Yamauchi Naoki
Faculty Of Agriculture Yamaguchi University:the United Graduate School Of Agricultural Sciences Tott
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Yamauchi Naoki
Faculty Of Agriculture Yamaguchi University
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Kaewsuksaeng Samak
Faculty of Agriculture, Yamaguchi University
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Funamoto Yoshio
The United Graduate School of Agricultural Sciences, Tottori University
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Shigyo Masayoshi
Faculty of Agriculture, Yamaguchi University
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Shigyo Masayoshi
Faculty Of Agriculture Yamaguchi University:the United Graduate School Of Agricultural Sciences Tott
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Funamoto Yoshio
The United Graduate School Of Agricultural Sciences Tottori University
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Kaewsuksaeng Samak
Faculty Of Agriculture Yamaguchi University:division Of Postharvest Technology School Of Bioresource
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